Table 3. Inhibitory activities of the purified proteins.

Purified N-TIMP2 mutants were assayed for their ability to inhibit MMP-14_CAT and MMP-9_CAT. K_i^app values were obtained from at least three such assay experiments for each protein and averaged, each value is reported as K_i^app ± S.D. The exceptions are a few experiments where very high mutant concentration was required and thus only one experiment was performed. The boldface values of each cell shows the relative inhibitory affinity for the target vs. off-target MMP: K_i^app for MMP-target/K_i^app for MMP-x, where target is MMP-14_CAT or MMP-9_CAT and x is any off-target MMP.

Kiapp of binding (nM)
	MMP-14CAT	MMP-9CAT	MMP-1CAT	MMP-2CAT	MMP-10CAT
WT	2.52±0.32	0.12±0.02	0.25±0.02	2.65±0.76	4.3±0.29
14D-4	1.02±0.62	1.28±0.18	0.31±0.03	83.96±4.57	78.91±41.55
	1.0	0.80	3.38	0.01	0.01
14D-6	0.89±0.06	2.43±0.90	0.78±0.21	224.5	446.35±167.09
	1.0	0.37	1.14	0.004	0.002
9D-2	105.73±4.78	0.15±0.04	0.11±0.02	3.53±0.34	>300
	0.001	1.0	1.36	0.04	<0.0005
9D-6	463.50±33.16	0.50±0.12	9.53±3.21	38.89±2.50	>300
	0.001	1.0	0.05	0.01	<0.002