Fig. 4.

Use of the BBWV-2 vector for gene expression and functional analysis in gentian. (A) Fluorescence produced by the green fluorescent protein (GFP-3FLAG, 819 nucleotides) in crowns at 162 days post inoculation (dpi). (B) Detection of β-glucuronidase (GUS, 1,809 nucleotides) activity by histochemical staining with X-Gluc in inoculated leaves at 17 dpi. (C) Reduced pigmentation in flower petals with a chimeric repressor responsible for flower pigmentation (GtMYB3-SRDX, 960 nucleotides) at 70 dpi. White arrows indicate the partial change of petal pigmentation from blue to white. Scale bar = 5 mm.