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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Environ Microbiol. 2017 Dec 22;20(4):1330–1349. doi: 10.1111/1462-2920.14020

Figure 3. R. solanacearum enriches putrescine in xylem sap and culture medium.

Figure 3

(A) Xylem sap was harvested at symptom onset from tomato plants (cv. Money Maker) infected with R. solanacearum strains; water-inoculated plants served as controls. Putrescine was measured by LC-MS. (B) Putrescine in spent culture supernatant was detected by growth of the GMI1000 ΔspeC mutant, a sensitive putrescine biosensor as shown in Figure 4. R. solanacearum strains were grown in minimal medium (MM) for 24 h. Culture supernatants were filter-sterilized and added to fresh MM at 10% v/v and inoculated with ΔspeC. Growth of ΔspeC mutant was measured at 24 h. Values are mean ± SEM. (*P<0.05 vs. healthy, t-test with Holm-Bonferroni correction, n≥3 pools).