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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Anesthesiology. 2018 May;128(5):967–983. doi: 10.1097/ALN.0000000000002063

Figure 2.

Figure 2

The number of Enkephalin (Enk-ir) and co-labeled mu-opioid receptor (mOR-ir) and GFP-positive epidermal nerve fibers in plantar hind paw skin increases after hsvMOR, hsvPPE and hsvMOR + PPE inoculation. A. Top: Quantification of Enk-ir positive nerve fibers of epidermis on Day 16 post viral inoculation. There is a significant increase in Enk-ir in hsvMOR, hsvPPE and hsvMOR + PPE treatment groups compared to sham and control virus (ANOVA, F(4, 19) = 2.3, P = 0.01; hsvCON). Bottom: Histology images of Enk+ epidermal nerve fibers from forepaw footpad skin following treatment with control virus (left) and hsvPPE (right). B. Top: Quantification of MOR-ir positive nerve fibers of epidermis on Day 16 post viral inoculation. Bottom: Histology images of MOR+ epidermal nerve fibers from the forepaw footpad skin following treatment with control virus (left) and hsvMOR (right). There is a significant increase in MOR-ir in hsvMOR, hsvPPE and hsvMOR + PPE treatment groups compared to sham (ANOVA, F(4, 19) = 3.5, P = 0.032). # vs. sham; # significant at P<0.05; ## significant at P<0.01. * vs. control virus- treated (hsvCON);* significant at P<0.05; ** significant at P<0.01. Bonferroni post-hoc for multiple comparisons.