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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Anesthesiology. 2018 May;128(5):967–983. doi: 10.1097/ALN.0000000000002063

Figure 5.

Figure 5

Mechanical allodynia and thermal hyperalgesia after L5 spinal nerve ligation surgery are attenuated in hsvMOR inoculated mice. At 7 days after nerve injury (arrow), MOR, PPE, MOR+PPE combination, or control (CON) virus was administered into the intraplantar aspect of the ipsilateral hind paw; mechanical allodynia and thermal hyperalgesia were then assessed over 15 days. (A) MOR and PPE virus infections reversed mechanical allodynia after 12 and 16 days, respectively. The MOR+PPE virus-infected mice had an allodynia reversal time course comparable to that of control virus-infected mice. Paw withdrawal threshold increased significantly over time for MOR virus-inoculated animals (repeated measures ANOVA, F(30, 500) = 3.9, *P<0.001 vs. control group. (B) The paw withdrawal threshold after MOR infection was significantly increased compared to control virus infection at the conclusion of the behavioral study (Day 22, ANOVA, F(3, 50) = 8.3, *P<0.001 vs. control group. (C) MOR and PPE virus infections reversed thermal allodynia after 12 and 16 days, respectively, compared to 21 days for control virus infection (CON). Mice administered the MOR+PPE virus combination did not have allodynia reversal during the time course of the experiment. (D) The thermal threshold after MOR infection was significantly increased compared with that after control virus infection at the conclusion of the behavioral study (Day 22, ANOVA, F(3, 50) = 5.9, *P = 0.022 vs. control group). Bonferroni post-hoc for multiple comparisons.