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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Am J Transplant. 2017 Nov 23;18(5):1096–1109. doi: 10.1111/ajt.14544

Figure 7. Rapamycin treatment inhibits activation of anti-HLA class I mAb-induced mTOR signaling proteins in vivo.

Figure 7

BALB/c cardiac allografts were transplanted into B6.RAG1−/− recipients, passively transfused with anti-MHC I or isotype control and treated with daily Rapa before immunohistochemical analysis of proteins involved in MHC I-induced cell survival/proliferation pathways. (A) Grafts were procured at day 30 post-transplant and evaluated for p-ERK, p-AKT, p-S6K and/or p-ERM expression by immunohistochemical staining. All phosphorylated proteins are visible in cardiac vessels and endocardium (dark brown) in cardiac vessels and endocardium. scale bars = 50 µm (C) Shown are mean scores ± SEM for n≥6 per group with each dot representing one animal. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 by two-way ANOVA with Tukey’s multiple comparisons test.