Table 2.
Formulation of Culture Media for Human Colonic Epithelial Cells
| Reagent | EM | SM | DM |
|---|---|---|---|
| WRN–conditioned medium | 50 vol% | 50 vol% | |
| Advanced DMEM/F12 | 50 vol% | 50 vol% | 100 vol% |
| GlutaMax | 1× | 1× | 1× |
| HEPES | 10 mmol/L | 10 mmol/L | 10 mmol/L |
| Primocin | 50 μg/mL | 50 μg/mL | 50 μg/mL |
| N-acetyl cysteine | 1.25 mmol/L | 1.25 mmol/L | 1.25 mmol/L |
| Murine EGF | 50 ng/mL | 50 ng/mL | 50 ng/mL |
| Nicotinamide | 10 mmol/L | ||
| B27 | 1× | ||
| Gastrin | 10 nmol/L | ||
| PGE2 | 10 nmol/L | ||
| A83-01 | 500 nmol/L | 500 nmol/L | |
| SB202190 | 3 μmol/L | ||
| Y27632 | 10 μmol/La |
DMEM, Dulbecco's modified Eagle medium; EGF, epidermal growth factor.
a
Used in the first 48 hours after cell plating to prevent dissociation-induced cell apoptosis.