Fig. 4.

Schematic and results of a PCR-based assay (in-out PCR). PCR-based assay used to detect targeted integration of the tag sequence in single-cell-derived HEK293T clones obtained by limiting dilution following CRISPR/Cas9-driven gene targeting. Primers are located outside of the homology arms and are designed to yield a PCR product if the tag is inserted. WT indicates untagged cells and 1–14 are single-cell-derived clones