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Western Blot analysis of single-cell-derived HEK293T clones. Western Blots showing HIS-tag (upper panel) and FLAG-tag protein expression in single-cell-derived HEK293T clones obtained by limited dilution following CRISPR/Cas9-driven gene targeting using HIS and FLAG antibody, α-tubulin is used as a loading control. WT indicates untagged cells, P indicates Hsc70 expressed on a plasmid, and 1–14 are single-cell-derived clones
