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CRISPR-mediated tagging of Hsc70 is isoform specific. a Amino acid and genomic DNA sequences of HSC70 and HSP72. The HSC70 gRNA binding site is absent in the HSP72 gene. b Luminespib induces Hsp72 levels but not HIS-Hsc70 levels. WT indicates untagged cells; clones 1–3 are three separate HEK293THIS-FLAG-Hsc70 clones. α-tubulin is used as a loading control in this instance
