Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Validation of known Hsc70 interactions in HEK293THIS-FLAG-Hsc70 cells. Protein extracts were obtained from WT and three separate HEK293THIS-FLAG-Hsc70 clones. Hsc70 complexes were purified using either HIS or FLAG magnetic beads. Hsc70 complexes were resolved by SDS-PAGE and analyzed by Western Blot using antibodies to co-chaperone proteins HSP27, HDJ2, and HSP110
