Fig. 3.

Effect of rhHsp70 and LTA combination on production of IL-8 after 24 h treatment. a Production of IL-8 after 24 h treatment with 1 μg/ml LTA alone and in combination with 0.3 (LTA + 0.3), 1 (LTA + 1), and 3 (LTA + 3) μg/ml rhHsp70. Data are presented as mean ± SEM and tested by ANOVA followed by multiple comparison Sidak method. *P = 0.0024 for LTA alone vs. non-treated cells. *P = 0.0002, P = 0.0005, and P = 0.0001, for combination of LTA with 0.3, 1, and 3 μg/ml rhHsp70, respectively vs. non-treated cells. **P = 0.0196 vs. LTA alone. b rhHsp70 and LTA interactions on IL-8 secretion. Dark bars represent the measured values and gray bars the expected values. Data are expressed as mean ± SEM. Tested using unpaired t test. Double number signs indicate statistically significant antagonistic effect; P = 0.0038 for combination of LTA with 3 μg/ml rhHsp70