Fig. 3.

Complementation of yeast scs2Δ mutant with TaVAP. Inositol auxotrophy of yeast scs2Δ mutant strain, scs2Δ mutant cells transformed with pYES2.0:TaVAP, and parental strain was determined by streaking the cells on either inositol-containing (INO+) or inositol-free (INO-) minimal synthetic dropout (SD) medium followed by incubation at 28 °C for 72 h or at 37 °C for 96 h. SD medium was added with galactose (2%) and raffinose (1%)