Alignment of the amino-terminal presequence of Arabidopsis ACX3 with other microbody proteins that are synthesized as larger precursors. AtACX3, Arabidopsis ACX3; AtACX2, Arabidopsis ACX2 (Hooks et al., 1999); PumLACX, pumpkin long-chain ACX (Hayashi et al., 1998a); PhaACX, Phalaenopsis sp. ACX (Do and Huang, 1997); PumMDH, pumpkin glyoxysomal MDH (Kato et al., 1998); PumCS, pumpkin glyoxysomal CS (Kato et al., 1995); AtPED1, Arabidopsis thiolase (Hayashi et al., 1998b); AtPKT2, Arabidopsis thiolase (Ferreira da Rocha et al., 1996); PumTHI, pumpkin glyoxysomal thiolase (Kato et al., 1996b). Conserved amino acids, where five of the nine sequences are identical are shown in a black box. The first amino acid, as determined by sequencing of amino-terminal residues of the mature proteins, are shown in a gray box.