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. 2000 Jun;123(2):733–742. doi: 10.1104/pp.123.2.733

Figure 5.

Figure 5

Northern-blot analyses of ACX3, SACX, PED1, and ICL in developing and germinating B. napus seeds. Each lane was loaded with 10 μg of total RNA isolated from either B. napus cotyledons (except for d 1, which included the entire seedling) or developing seeds. The developmental stage are shown as day after imbibition for the germinating seed or DPA for the developing seed. Blots were probed with: AtACX3, Arabidopsis ACX3; AtSACX, Arabidopsis SACX (Hayashi et al., 1999); PED1, Arabidopsis thiolase (Hayashi et al., 1998b); ICL, B. napus ICL (accession no. Y13356). The ethidium bromide-stained ribosomal RNA bands are show as a loading control.