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. 2018 Apr 17;9:1513. doi: 10.1038/s41467-018-03986-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — The PI3Kγ signaling is enhanced in the human heart tissue with Chagas disease. a Transcriptome analysis of family I PIK3 genes expression (PIK3CA, PIK3CB, PIK3CD, and PIK3CG) in healthy control (Ctl; n = 7) and patients with idiopathic dilated cardiomyopathy (DCM; n = 14) or chronic chagasic cardiomyopathy (CCC; n = 10). b RT-PCR analysis of the mRNA expression of PIK3CA, PIK3CB, PIK3CD, and PIK3CG genes in the heart tissue of Ctl (n = 5); DCM (n = 10); and CCC (n = 10) patients. GAPDH was used as a housekeeping gene. c Representative western blots and analysis of phosphorylated (p) and total (t) AKT1 expression in the heart tissue of Ctl (n = 5), DCM (n = 10), and CCC (n = 10) patients. GAPDH was used as a loading control. d Representative western blots and analysis of phosphorylated (p) and total (t) AKT2 expression in the heart tissue of Ctl (n = 5), DCM (n = 10), and CCC (n = 10) patients. GAPDH was used as a loading control. AU refers to arbitrary units. ns = no statistical significance (one-way ANOVA with Tukey’s post hoc test in b–d). *P < 0.05 and **P < 0.01 relative to Ctl and #P < 0.05, ##P < 0.01, and ###P < 0.001 relative to DCM (one-way ANOVA with Tukey’s post hoc test in b–d). Data are from one experiment (mean ± s.e.m in b–d)