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. 2018 Apr 17;8:6065. doi: 10.1038/s41598-018-24531-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treg EV derived miRNAs are transferred into DCs. (A) Bar graphs show relative quantity (RQ) of miR-142-3p and miR-150-5p in untreated B6 derived DCs (DC), control T cell EV-treated DCs (DC+Control EVs), or Treg EVs-treated DCs (DC+Treg EVs). DCs were treated with EVs for 24 hours, washed thoroughly and miRNA presence was measured by qPCR normalised to RNU6-2. Data represents mean+SEM pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using One-way ANOVA and Tukey’s multiple comparison test, ****p < 0.0001. (B) Bar graphs show RQ of miR-142-3p and miR-150-5p in untreated Dicer deficient-derived DCs (DC), control T cell EVs-treated Dicer-deficient DCs (DC+Control EVs) or Treg EVs-treated Dicer-deficient DCs (DC+Treg EVs). Dicer-deficient DCs were treated with EVs for 24 hours, washed thoroughly and miRNA presence was measured by qPCR and normalised to RNU6-2. Data shown is mean+SEM representative of 2 experiments that were performed in technical triplicates. Statistical significance was determined using One-way ANOVA and Tukey’s multiple comparison test, ****p < 0.0001 and ns = not significant.