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. 2018 Apr 17;8:6071. doi: 10.1038/s41598-018-24440-w

Figure 1.

Figure 1

Analysis of IR-induced apoptosis in different fractions of primitive human hematopoietic cells. (A) Flow cytometry gating strategy for HSPCs and CPs. (B,D) Example of FACS data identifying cPARP+ (B) and AnnexinV+ (D) fractions in HSPCs and CPs gated as described in (A) 6 h after 3 Gy IR-treatment. (C,E) CD34+ cells were pre-treated with Z-vad-FMK (100 μM), Q-VD-OPh (20 μM) or DMSO for 1 h, irradiated with 3 Gy and analyzed for cPARP+ or Annexin V+ cells in HSPCs and CPs 6 h later, n ≥ 3. (F) Survival of clonogenic cells from sorted HSPC (CD34+CD38−/lowCD90+45RA−), CMP (CD34+CD38+CD45RA−CD135+), GMP (CD34+CD38+CD45RA+CD135+) and MEP (CD34+CD38+CD45RA−CD135−) fractions following IR with 3 Gy. CFC surviving fraction was calculated by dividing number of colonies counted in the irradiated plates by the number of colonies from the same fraction scored in the non-irradiated plates, n = 3. Represented are mean values ± SD; *p < 0.05, **p < 0.01, ***p < 0.001.