Fig. 5.

Incorporation of ¹³C methanol during exponential growth. a Illustration of biomass formation from unlabeled gluconate and ¹³C methanol. b Isotopologue distribution of hexose 6-phosphate in an E. coli wild-type strain containing mdh and hps phi (top) and MeSV2.2 (bottom). c Labeling detected in MeSV2.2 without yeast extract (closed squares) and MeSV2.1 grown with additional 0.1 g L⁻¹ unlabeled yeast extract (closed circles) are shown. For comparison, the results of E. coli wild type containing mdh and hps phi without yeast extract (open squares) and with 0.1 g L⁻¹ unlabeled yeast extract (open circles) are provided. Data presented as mean ± standard deviation; n = 3. The red dotted line represents the expected labeling fraction under equimolar consumption of gluconate and methanol