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. 2018 Apr 17;9:1508. doi: 10.1038/s41467-018-03937-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Prominent mutated genes in evolution experiments. a The fraction of mutations observed in independent evolution experiments is shown in brackets. Number of sequenced populations: MeSV1.1 n = 5, MeSV2.1 n = 6, MeSV2.2 n = 1. Mutations in frmA and gntR appear in all experiments whereas mutations in nadR and rho are only seen in MeSV2 strains. b Prominent mutated genes encoding enzymes in close proximity to branch points of the synthetic RuMP cycle are shown. For a detailed description of all mutations, see Supplementary Data 3