Table 3.
Characteristics of CMs from male NTL and dn-c-kit-Tg mice (age P112-P121)
| Parameter | NTL | Tg |
|---|---|---|
| % 1N | 10 ± 1 (4) | 12 ± 2 (4) |
| % 2N | 79 ± 2 (4) | 74 ± 2 (4) |
| % >2N | 11 ± 1 (4) | 14 ± 3 (4) |
| % 2n | 96.5 ± 0.3 (4) | 96.2 ± 0.3 (6) |
| % 4n | 3.5 ± 0.6 (4) | 3.7 ± 0.5 (6) |
| % >4n | 0.05 ± 0.01 (4) | 0.1 ± 0.07 (6) |
| %BrdU+/cTnT+ CMs | 0.013 ± 0.01 (2) | 0.301 ± 0.07** (2) |
| %H3P+/cTnT+ CMs | 0 (2) | 0.017 ± 0.008 (2) |
| %AurB+/cTnT+ CMs | 0 (2) | 0 (2) |
| TUNEL staining | 0 (2) | 0 (2) |
| CM area, μm2 | 4898 ± 52 (3) | 6112 ± 78* (3) |
| No. of CMs/heart | 2.2 × 106 ± 1 × 105 (7) | 2.1 × 106 ± 3 × 104 (9) |
Data are presented as mean ± SEM. N, CM nucleation state: mono (1N), bi (2N), multi-nucleated (>2N) determined from 3,699 NTL CMs and 3,193 Tg CMs from n = 4 mice; % n, haploid number in CM nuclei (ploidy state) determined from 42,589 NTL and 57,676 Tg CMs from n = 4 and n = 6 mice, respectively; 115,070 NTL CMs and 88,473 Tg CMs from n = 2 mice were analysed for BrdU-positivity; 85,000 NTL CMs and 86,400 Tg CMs from n = 2 mice were analysed for H3P-positivity; 68,000 NTL CMs and 72,000 Tg CMs from n = 2 mice were analysed for AurB-positivity; 1015 NTL CMs and 682 Tg CMs from n = 3 mice were analysed for CM area. Student’s t-test: **p < 0.01; ***p < 0.001.