Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 17;9:1514. doi: 10.1038/s41467-018-03803-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — GOT2 supports the proliferation of IL10 + CpG-stimulated and MYC-overexpressing cells. a Transient knockdown of GOT1 and GOT2 in P493-6 cells was performed by siRNA transfection. Knockdown efficiencies (GOT*/tubulin) relative to scrb control are provided under the images. b Relative cell numbers of IL10 + CpG-stimulated P493-6 MYC^low and P493-6 MYC^high after GOT1 or GOT2 knockdown depicted in a. Relative cell numbers of c IL10 + CpG-stimulated P493-6 MYC^low and d unstimulated P493-6 MYC^high cells after GOT2 knockdown and indicated α-KG, Asp, A or T addition as described in Fig. 2. e Transient knockdown of GPT2 in P493-6 cells performed by siRNA transfection. Knockdown efficiencies (GPT2/tubulin) relative to scrb control are provided under the images. f Relative cell numbers of IL10 + CpG-stimulated P493-6 MYC^low and P493-6 MYC^high cells after GPT2 knockdown as depicted in e. All experiments were performed in triplicates. For b–d and f, error bars represent mean ± SD of three independent experiments and results from Bonferroni post hoc tests on a one-way ANOVA are given (*p < 0.05, **p < 0.01, ***p < 0.001)