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. 2018 Apr 17;8:6112. doi: 10.1038/s41598-018-24394-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Longevity of the cross-reactive CD8 T cell response. Naïve ferrets were infected intranasally with H1N1pdm09 (H1N1pdm09, n = 7), or mock infected (Mock; n = 6). Peripheral blood leukocytes were purified weekly for 6 weeks, and CD8 T cell responses assessed by stimulation with live virus consisting of a panel of contemporary and historical influenza strains (A). Splenocytes were collected 6 weeks post-infection and stimulated with the panel of influenza strains (B). Responding CD8 + IFN-γ + T cells were assessed by flow cytometry. Significance from the mock infected group is indicated by *p < 0.05, ^∇p < 0.01, ^†p < 0.001. Error bars represent SEM.