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. 2018 Apr 17;8:6097. doi: 10.1038/s41598-018-24309-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SlUVR8 enhances SlGLK2 accumulation under UV radiation. (A) Immunoblot analysis of SlGLK2 levels among Ailsa Craig (WT), 35S::SlUVR8Ri and 35S::SlUVR8OE transgenic lines. The proteins extracted from immature fruits (25 DPA) from field-grown plants was resolved by SDS-PAGE, then probed with anti-SlGLK2 antibody and anti-β-actin antibodies. (B) Immunoblot analysis of SlGLK2 levels between Micro Tom (WT) plant grown in white light and plant grown in white light with added 3 days of UV-B irradiation. The proteins extracted from immature fruits (15 DPA) was resolved by SDS-PAGE, then probed with anti-SlGLK2 antibody and anti-β-actin antibodies. Blots were quantitatively analyzed by software ImageJ 1.46r. Values were shown as “means ± SD” from results of three independent experiments. (C) Quantitative RT-PCR analysis of SlGLK2 mRNA levels in immature fruits (15 DPA) from Micro Tom (WT) with different UV-B irradiation time (0 d, 1 d and 3 d respectively). Values were shown as “means ± SD”; Error bars represent SD of 3 biological replicates. “*” and “**” means P < 0.05 and P < 0.001 respectively (Student’s t test). (D) Quantitative RT-PCR analysis of chloroplast development related genes SlGLK2, SlPsaL and SlPsbQ in Ailsa Craig (WT), 35S::SlUVR8Ri and 35S::SlUVR8OE transgenic lines. Total RNAs were extracted from fruit pericarps of immature fruits (25 DPA) from field-grown plants. Values were shown as “means ± SD”; Error bars represent SD of 3 biological replicates. “*” and “**” means P < 0.05 and P < 0.001 respectively (Student’s t test).