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. 2018 Apr 17;8:6097. doi: 10.1038/s41598-018-24309-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SlUVR8 is required for UV-induced SlGLK2 expression. (A) Quantitative RT-PCR analysis of chloroplast development related genes SlGLK2 and its target genes (SlPsaL and SlPsbQ) in Ailsa Craig (WT), 35S::SlUVR8OE and 35S::SlUVR8Ri transgenic lines with or without 3-d UV-B irradiation. Total RNAs were extracted from totally expanded leaves of 30-d old seedlings grown in white light. Values were shown as “means ± SD”; Error bars represent SD of 3 biological replicates. “*” and “**” means P < 0.05 and P < 0.001 respectively (Student’s t test). (B) Quantitative RT-PCR analysis of SlGLK1 gene in the same samples as described in (A). “**” means P < 0.001 (Student’s t test).