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. 2018 Apr 11;9:670. doi: 10.3389/fmicb.2018.00670

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Copyright © 2018 González, Vázquez, Cullen, Mas, Beltran and Torija.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Invasive growth phenotypes of different wine yeast species. (A) In a plate washing assay (PWA), equal concentrations of cells were spread on media with different nutrient contents and incubated for 5 days at 28°C. (B) Quantification of invasive growth was performed after washing the plate via densitometry analysis. Cells were spotted in triplicate, and the average values are shown. Statistical analysis was carried out by comparing each strain with respect to rich media (SAD), using Tukey’s test statistical method; asterisk denotes a p-value < 0.05.