Table 1.

Primer features of eight candidate reference genes for RT-qPCR in S. litura.

Gene name (Abbreviation)	Accession No.	Primer Sequence	Product length (bp)	Tm (°C)	Primer efficiency (%)	R2	Slope
RpL13A		5′ TGTGGAAGACTGTCAGAGGCA 3′	106	60	97.2	0.998	−3.391
		5′ GGTTGTCATAGGGTGGTGGG 3′
alpha-tubulin		5′ CAGATGCCCTCCGACAAGA 3′	113	60	92.4	0.999	−3.518
		5′ GGCTCCAAGTCCACGAACA 3′
RpL7A		5′ CGCCCTTTGCCGTAAGAT 3′	222	60	95.3	0.997	−3.439
		5′ TTGTTGCCGAGGACACCAC 3′
Actin	KP331524.1	5′ GTTGCTGCGTTGGTAGTAGACA 3′	202	60	95.5	0.999	−3.435
		5′ CGATGGGGTACTTGAGGGTAA 3′
GAPDH	HQ012003.2	5′ TTGATGGACCCTCTGGAAAAC 3′	154	60	95.0	0.998	−3.448
		5′ TTAGCAACAGGAACACGGAAA 3′
EF2		5′ ACCAAGAAGTGGGCGAAACA 3′	214	60	94.0	0.999	−3.473
		5′ GCAGCCAGCTACGCATAACC 3′
EF1- alpha	KC007373.1	5′ CCCTGCCAACATCACCACT 3′	131	60	94.4	0.986	−3.464
		5′ CGTAACCACGACGCAACTCC 3′
RpL10A		5′ CTCATCAAGCAGATCCCACG 3′	160	60	93.6	1.000	−3.486
		5′ CAGCCACAGACAGGCACAATA 3′
18S ribosomal RNA	JX041463.1	5′ CCTGCTAAATAGGCGTCGTCA 3′	225	60	93.5	0.998	−3.488
		5′ AGGAGTTTCAGCGGGTTGC 3′
beta-tubulin		5′ TTGCCTCCAAGGATTCCAAC 3′	135	60	101.1	0.995	−3.296
		5′ GGCGAAGGTACAACTGAGTATGTG 3′