Figure 2.

Modulation of large-scale Min protein patterns by MinE activity and concentration. (a) ATPase stimulation assay with WT MinE and MinE K19Q and R21A using 4 µM MinD, 4 µM MinE and 0.2 mg ml⁻¹ small unilamellar vesicles made of E. coli polar lipids. Error bars represent standard deviations (N = 3). (b) Schematic of the self-organization assay on flat SLBs. (c) Confocal images of the self-organization assay at different MinE concentrations with MinD constant at 1 µM with 20% eGFP-MinD. Scale bars, 50 µm. Dependence of the mean wavelength (d) and velocity (e) of WT and K19Q waves on MinE concentration (MinD at 1 µM). Error bars represent standard deviations (N ≥ 7 waves from three independent experiments).