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. 2018 Apr 11;9:615. doi: 10.3389/fimmu.2018.00615

Figure 4.

Figure 4

Effects of Met-RANTES treatment on splenomegaly, myocardial injury, and CCL5 and TNF concentrations in the heart tissue of T. cruzi-infected mice. (A) Mice were infected with 100 trypomastigote forms of the Colombian T. cruzi strain and treated with Met-RANTES (Met-R, 10 µg/mice) from 120 to 150 days postinfection (dpi). Sera were collected (at 120 and 150 dpi) for estimation of CK-MB activity. Hearts were collected (at 150 dpi) for evaluation of CCL5 and TNF concentrations by enzyme-linked immunosorbent assay (ELISA). (B) The relative weight of the spleen (spleen weight in milligrams/body weight in grams) at 150 dpi. (C) CK-MB activity in serum determined by biochemical assay at 120 (pretherapy) and 150 dpi (posttherapy, Met-R). (D) CCL5 concentrations in extracts of heart tissue evaluated by ELISA at 150 dpi. (E) TNF concentrations in extracts of heart tissue evaluated by ELISA at 150 dpi. Data represent two independent experiments with 3–5 noninfected (NI) and 7–10 infected mice.*P < 0.05, **P < 0.01, ***P < 0.001, T. cruzi-infected mice vs NI; #P < 0.05, ##P < 0.01, and ###P < 0.001, pretreated vs Met-R-treated; &&P < 0.01, &&&P < 0.001, Ccr5−/− vs Ccr5+/+ T. cruzi-infected mice pretreatment (120 dpi); ψP < 0.05, ψψP < 0.01, ψψψP < 0.001, Ccr5−/− vs Ccr5+/+ T. cruzi-infected mice posttreatment (150 dpi) with Met-R (analysis of variance, Bonferroni posttest).