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. 2018 Apr 11;9:476. doi: 10.3389/fpls.2018.00476

FIGURE 2.

FIGURE 2

OsCCX2 structure analysis and ccx2 mutant generation. (A) Targeted mutagenesis of OsCCX2 gene by CRISPR-Cas9. Two independent gene edition sites were designed (NGG motifs underlined). Sequences of the mutant alleles are aligned to the genome sequence of wild type, and two homozygous mutant lines (ccx2-1 and ccx2-2) were obtained with 1 bp insertion and 1 bp deletion separately (shown by red arrows). (B) Schematic topology diagrams of OsCCX2. OsCCX2 protein with 12 transmembrane domains and two Na/Ca exchanger domains. (C,D) Seedlings of two homozygous mutant lines (ccx2-1 and ccx2-2). The germinated seeds were grown in hydroponic solution for 4 weeks (C), and the lengths of roots and shoots of the wild type and mutant plants were measured (n = 20 for each data point) (D). Data are average values of three independent experiments and are presented as mean ± SD. Bar = 3 cm (C).