FIGURE 8.

Subcellular localization of OsCCX2. The OsCCX2 coding sequence was fused to the N terminus of the GFP coding region in the pCambia2300 and pCambia1302 vector separately, and then transformed into Arabidopsis mesophyll protoplasts and wild type Nipponbare, respectively. The empty vector pCambia2300-transformed protoplasts were used as the control. The fluorescent signals were imaged by using an LSM710 confocal laser scanning microscope. Bars = 10 μm. (A) OsCCX2-GFP signal in Arabidopsis mesophyll protoplasts. The green signals were from GFP, and the red signals were from chloroplasts. (B) OsCCX2-GFP signal in root tip cells of Nipponbare rice. The green fluorescence came from GFP signals, and the red signals were from plasma membrane-specific fluorescent dye FM4-64. The yellow signals were merges of the green and red signals.