Table 1.
DNA content and size, CMA3+ bands and distribution of the in situ hybridization signals (GISH and FISH) on chromosomes of the four Arachis genotypes.
| Genotypes | A. duranensis | A. ipaensis | IpaDur1 | A. hypogaea | |
|---|---|---|---|---|---|
| Karyotype formula | 9 m + 1 sm | 9 m + 1 sm | 18 m + 2 sm | 18 m + 2 sm | |
| DNA content (2C) (pg) | 2.62 | 3.34 | 5.92 | 5.70 | |
| Size (1C) (Gb | 1.28 | 1.63 | 2.89 | 2.79 | |
| CV (%) | 2.67 | 4.14 | 2.36 | 3.25 | |
| CMA 3 + | Proximal regions on cyt-A10* | Proximal region on cyt-B10* | Proximal region on cyt-A10* and cyt-B10 | Proximal regions on cyt-A10*, cyt-B10 and another three pairs | |
| GISH (genomic probes) | IpaDur1 | – | – | On all chromosomes, for both subgenomes. Few signals on centromeres of A subgenome chromosomes and terminal regions. Cyt-B10 entirely covered by signals | On all chromosomes, for both subgenomes. Seldom signals on cyt-A9. Few signals on centromeres of A subgenome chromosomes and terminal regions. Cyt-B10 entirely covered by signals |
| A. hypogaea | – | – | On all chromosomes, for both subgenomes. Seldom signals on cyt-A9. Few signals on centromeres of A subgenome chromosomes and terminal regions. Cyt-B10 with alternated pattern | On all chromosomes, of both subgenomes. Seldom signals on cyt-A9. Few signals on centromeres of A subgenome chromosomes and terminal regions. Cyt-B10 entirely covered by signals | |
| A. duranensis and A. ipaensis | – | – | Higher affinity to chromosomes of each corresponding subgenome. Hybridized poorly on cyt-A9, centromeres of A chromosomes and terminal regions of all chromosomes. Cyt-B10 with mosaic pattern | Higher affinity to chromosomes of each corresponding subgenome. Hybridized poorly on cyt-A9, centromeres of A chromosomes and terminal regions of all chromosomes. Cyt-B10 with higher affinity to A. ipaensis probe | |
| rDNA FISH | 5S | Proximal region on cyt-A3 | Proximal region on cyt-B3 | Interstitial region on cyt-A3 and proximal region on cyt-B3 | Interstitial region on cyt-A3 and proximal region on cyt-B3 |
| 45S | Proximal region on cyt-A2 and A10* | Proximal region on cyt-B3 and B10* and on terminal region on cyt-B7 | Proximal region on cyt-A2; A10* and B10 | Proximal regions on cyt-A2; A10*; B3 and B10 and in terminal regions on cyt-B7 | |
| LTR-RT FISH | RE128-84 | Dispersed on arms and proximal regions of all chromosomes. Seldom detected on centromeric and terminal regions | Dispersed on the arms and proximal regions of most chromosomes. Lacking on two pairs. Seldom detected on centromeric and terminal regions | Dispersed on the arms and proximal regions of most chromosomes. Lacking on one pair of chromosome of the subgenome A Seldom detected on centromeric and terminal regions | Dispersed on arms and proximal regions of most chromosomes. Lacking on cyt-A9 and cyt-A10. Seldom detected on centromeric and terminal regions |
| LTR-RT FISH | Pipoka | Dispersed on arms and proximal regions of most chromosomes. Poorly on cyt-A9 and cyt-A10. Seldom detected on centromeric and terminal regions | Dispersed on the arms and proximal regions of most chromosomes. Seldom detected on centromeric and terminal regions | Dispersed on the arms and proximal regions of most chromosomes. Lacking on cyt-A9, cyt-A10 and on two pairs of A subgenome. Seldom detected on centromeric and terminal regions | Dispersed on the arms and proximal regions of few chromosomes. Lacked on cyt-A9, cyt-A10. Seldom detected on centromeric and terminal regions |
| Athena | Dispersed on arms and proximal regions of most chromosomes. Seldom on centromeric and terminal regions | Dispersed on the arms and proximal regions of most chromosomes Lacking on terminal regions of all chromosomes | Dispersed on the arms and proximal regions of most chromosomes on B subgenome. Lacking on cyt-A9 and cyt-A10. Seldom detected on centromeric and terminal regions | Dispersed on the arms and proximal regions of most chromosomes, Lacking on cyt-A9 and cyt-A10. Seldom detected on centromeric and terminal regions |
CV: coefficient of variance; m: metacentric; sm: submetacentric; *: NOR (Nucleolar Organizing Region); –: not analysed.