TABLE 1 .
PCR detection of bacterial targets in individual micea
| Bacterium | Target gene | % prevalence by PCR at site: |
% of positive mice by: |
Total (%) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sex |
Age |
|||||||||||||
| M2 | M3 | Q1 | X1 | X2 | X3 | K1 | M | F | J | S | A | |||
| C. difficile | tcdB | 7/42 (16.7) | 2/119 (1.7) | 9/210 (4.3) | 0/38 (0.0) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 8/18 (44) | 10/18 (56) | 13/18 (72) | 0/18 (0) | 5/18 (28) | 18/416 (4.3) |
| C. perfringens | cpa | 8/42 (19.0) | 14/119 (11.8) | 25/210 (11.9) | 1/38 (2.6) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 24/48 (50) | 24/48 (50) | 18/48 (38) | 5/48 (10) | 25/48 (52) | 48/416 (11.5) |
| Salmonella spp. | invA | 2/42 (4.8) | 2/119 (1.7) | 9/210 (4.3) | 0/38 (0.0) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 6/13 (46) | 7/13 (54) | 7/13 (54) | 2/13 (15) | 4/13 (31) | 13/416 (3.1) |
| Shigella/EIEC | ipaH | 4/42 (9.5) | 23/119 (19.3) | 33/210 (15.7) | 0/38 (0.0) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 33/60 (55) | 27/60 (45) | 30/60 (50) | 12/60 (20) | 18/60 (30) | 60/416 (14.4) |
| ETEC | st | 0/42 (0.0) | 0/119 (0.0) | 0/210 (0.0) | 0/38 (0.0) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/416 (0.0) |
| EPEC (a/t) | eae | 2/42 (4.8) | 3/119 (2.5) | 12/210 (5.7) | 1/38 (2.6) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 12/18 (67) | 6/18 (33) | 9/18 (50) | 3/18 (17) | 6/18 (33) | 18/416 (4.3) |
| EPEC (t) | bfpA | 0/42 (0.0) | 0/119 (0.0) | 0/210 (0.0) | 0/38 (0.0) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/416 (0.0) |
| EAEC | aggR | 0/42 (0.0) | 5/119 (4.2) | 0/210 (0.0) | 0/38 (0.0) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 2/5 (40) | 3/5 (60) | 1/5 (20) | 0/5 (0) | 4/5 (80) | 5/416 (1.2) |
| STEC | stx1 | 0/42 (0.0) | 0/119 (0.0) | 0/210 (0.0) | 0/38 (0.0) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/416 (0.0) |
| STEC | stx2 | 0/42 (0.0) | 1/119 (0.8) | 0/210 (0.0) | 0/38 (0.0) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 1/1 (100) | 0/1 (0) | 1/1 (100) | 0/1 (0) | 0/1 (0) | 1/416 (0.2) |
| K. pneumoniae | 16S rRNA/khe | 4/42 (9.5) | 6/119 (5.0) | 25/210 (11.9) | 4/38 (10.5) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 21/39 (54) | 18/39 (46) | 8/39 (20) | 10/39 (26) | 21/39 (54) | 39/416 (9.4) |
| Leptospira spp. | 16S rRNA | 2/42 (4.8) | 6/119 (5.0) | 4/172 (2.3) | 2/38 (5.3) | 0/1 (0.0) | 0/1 (0.0) | 0/5 (0.0) | 11/14 (79) | 3/14 (21) | 3/14 (21) | 2/14 (14) | 9/14 (64) | 14/378 (3.7) |
a
Abbreviations: A, adult; a, atypical; EAEC, enteroaggregative E. coli; EIEC, enteroinvasive E. coli; EPEC, enteropathogenic E. coli; ETEC, enterotoxigenic E. coli; F, female; J, juvenile; M, male; SA, subadult; STEC, Shiga toxin-producing E. coli; t, typical.