Table 2.
CT values determined for neat and pooled EDTA blood samples.
|
Hofmann et al. (2008) assay; mean CT value ± SD (number positive) |
VetMAX assay; mean CT value ± SD (number positive) |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| BTV serotype | dpi | Neat | Pooled 1:2 | Pooled 1:5 | Pooled 1:10 | Pooled 1:20 | Neat | Pooled 1:2 | Pooled 1:5 | Pooled 1:10 | Pooled 1:20 |
| BTV-1 | 0 to 2 | 35.39 ± 0.59 (2/3) | 36.06 ± 0.87 (4/10) | 37.93 ± 0.53 (3/10) | Undet. ± n.a. (0/10) | n.d. ± n.a. (n.d.) | 35.35 ± 0.45 (2/3) | 37.94 ± 1.34 (5/10) | Undet. ± n.d. (0/10) | 38.03 ± n.a. (1/10) | n.d. ± n.a. (n.d.) |
| 3 to 6 | 33.26 ± 0.38 (3/3) | 34.57 ± 1.00 (10/10) | 36.1 ± 1.30 (7/10) | 38.04 ± 1.54 (5/10) | 37.76 ± n.a. (1/10) | 32.87 ± 0.33 (3/10) | 34.76 ± 0.80 (9/10) | 36.16 ± 1.34 (9/10) | 37.69 ± 0.84 (7/10) | 37.75 ± n.a. (1/10) | |
| 7 to 12a | 27.25 ± 0.01 (3/3) | 28.47 ± 0.25 (10/10) | 29.98 ± 0.22 (10/10) | 31.3 ± 0.22 (10/10) | 33.13 ± 0.82 (10/10) | 26.93 ± 0.09 (3/3) | 27.96 ± 0.10 (10/10) | 29.47 ± 0.12 (10/10) | 30.75 ± 0.18 (10/10) | 32.63 ± 0.80 (10/10) | |
| 13 to 30 | 30.33 ± 0.15 (3/3) | 31.27 ± 0.21 (10/10) | 33.19 ± 0.47 (10/10) | 35.11 ± 1.51 (10/10) | 37.46 ± 3.21 (10/10) | 29.67 ± 0.37 (3/3) | 31.03 ± 0.16 (10/10) | 32.64 ± 0.39 (10/10) | 35.09 ± 0.92 (10/10) | 35.84 ± 1.04 (10/10) | |
| BTV-4 | 0 to 2 | 37.65 ± 3.36 (3/3) | 38.92 ± n.a. (1/10) | 39.66 ± n.a. (1/10) | Undet. ± n.a. (0/10) | n.d. ± n.a. (n.d.) | 37.83 ± 1.00 (3/3) | 37.11 ± 0.80 (6/10) | 37.51 ± 0.75 (4/10) | Undet. ± n.a. (0/10) | n.d. ± n.a. (n.d.) |
| 3 to 6 | 32.67 ± 0.84 (3/3) | 35.19 ± 1.41 (10/10) | 37.55 ± 1.33 (3/10) | 36.15 ± 1.34 (3/10) | 37.09 ± n.a. (1/10) | 33.28 ± 0.23 (3/3) | 34.45 ± 1.13 (10/10) | 35.63 ± 0.95 (9/10) | 36.05 ± 1.25 (9/10) | 37.19 ± 0.66 (6/10) | |
| 7 to 12a | 25.53 ± 0.10 (3/3) | 26.24 ± 0.20 (10/10) | 27.92 ± 0.17 (10/10) | 28.97 ± 0.32 (10/10) | 30.52 ± 0.76 (10/10) | 26.61 ± 0.70 (3/3) | 27.56 ± 0.08 (10/10) | 28.97 ± 0.24 (10/10) | 30.21 ± 0.41 (10/10) | 31.35 ± 0.51 (10/10) | |
| 13 to 30 | 28.63 ± 0.35 (3/3) | 29.84 ± 0.38 (10/10) | 31.32 ± 0.41 (10/10) | 33.4 ± 1.32 (10/10) | 34.49 ± 0.96 (10/10) | 29.6 ± 0.20 (3/3) | 30.8 ± 0.40 (10/10) | 32.06 ± 0.34 (10/10) | 33.86 ± 0.80 (10/10) | 34.37 ± 0.79 (10/10) | |
| BTV-8 | 0 to 2 | 34.34 ± 0.29 (3/3) | 35.59 ± 1.21 (4/10) | 36.54 ± 1.05 (3/10) | 36.63 ± 0.90 (2/10) | n.d. ± n.a. (n.d.) | 37.05 ± 0.32 (2/3) | 37.86 ± 0.92 (8/10) | 37.52 ± 1.56 (6/10) | 38.74 ± 0.01 (2/10) | n.d. ± n.a. (n.d.) |
| 3 to 6 | 34.38 ± 0.70 (3/3) | 35.63 ± 0.76 (8/10) | 36.81 ± 0.52 (2/10) | 35.39 ± 0.71 (3/10) | 37.55 ± n.a. (1/10) | 32.87 ± 1.05 (3/3) | 33.76 ± 0.80 (10'10) | 35.52 ± 1.54 (10/10) | 35.86 ± 1.39 (7/10) | 37.01 ± 0.42 (3/10) | |
| 7 to 12a | 27.94 ± 0.24 (3/3) | 29.13 ± 0.14 (10/10) | 30.62 ± 0.54 (10/10) | 31.1 ± 0.40 (10/10) | 32.53 ± 0.52 (10/10) | 28.92 ± 0.17 (3/3) | 30.32 ± 0.21 (10/10) | 32 ± 0.65 (10/10) | 32.64 ± 0.46 (10/10) | 34.18 ± 0.81 (10/10) | |
| 13 to 30 | 31.38 ± 0.29 (3/3) | 32.11 ± 0.41 (10/10) | 33.8 ± 1.22 (10/10) | 35.16 ± 1.15 (10/10) | 36.23 ± 2.70 (08/10) | 30.45 ± 0.27 (3/3) | 31.25 ± 0.81 (10/10) | 33.15 ± 1.02 (10/10) | 34.86 ± 1.84 (9/10) | 36.63 ± 1.81 (9/10) | |
dpi. Days post infection, n.d. no data available, n.a. not applicable.
a
Peak viraemia.