Table 3.

Estimated probability (%) of detection for different pooling ratios dependent on the C_T value of a single positive sample.

		Probability (%) of detection of a single positive animal
Assay	Pooling ratio	CT 27	CT 30	CT 33	CT 36
		7–12 dpi	13–30 dpi	3–6 dpi	0–2 dpi
Hofmann	neat	100	99.94	98.69	76.14
	1:2	99.98	99.61	91.58	31.48
	1:5	99.92	98.13	68.86	8.54
	1:10	99.78	94.95	44.25	3.24
	1:20	99.08	82.02	16.16	0.81
VetMAX	neat	100	99.99	99.65	92.26
	1:2	100	99.90	97.60	63.19
	1:5	99.98	99.49	89.21	25.88
	1:10	99.94	98.60	74.79	11.13
	1:20	99.75	94.46	41.87	2.95