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. 2008 Apr 17;7(2):63–74. doi: 10.1111/j.1447-0578.2008.00202.x

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© 2008 The Authors. Journal compilation © 2008 Japan Society for Reproductive Medicine

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Effects of progesterone (P) on hamster sperm hyperactivation. (a) Significant increase with 20 ng/mL P. Values are means ± standard deviation. Modified Tyrode's albumin lactate pyruvate (mTALP) + 0.1% ethanol (EtOH) (vehicle, ), mTALP + 20 ng/mL P + 0.1% EtOH (P; ) and mTALP + 20 ng/mL P + 23.4 µmol/L RU486 + 0.1% EtOH (P and RU486, ). ^aSignificantly different from vehicle (P < 0.01); ^bsignificantly different from P and RU486 (P < 0.01). (b) Sperm hyperactivation significantly increased by P in a concentration‐dependent manner. Values are means ± standard deviation. Vehicle (), 10 ng/mL P (), 20 ng/mL P () and 40 ng/mL P (). ^aSignificantly different from vehicle (P < 0.01); ^bsignificantly different from 10 ng/mL P (P < 0.01); ^csignificantly different from 40 ng/mL P (P < 0.01); ^dsignificantly different from vehicle (P < 0.05). (c) Sperm hyperactivation increased by non‐genomic signals of P. Values are means ± standard deviation. Vehicle (), 20 ng/mL P (), mTALP + 7 nmol/L fluorescein isothiocyanate and bovine serum albumin conjugated progesterone + 0.1% EtOH (FITC/BSA‐P, ) and mTALP + 7 nmol/L FITC/BSA‐P + 23.4 µmol/L RU486 + 0.1% EtOH (FITC/BSA‐P and RU486, ). ^aSignificantly different from vehicle (P < 0.01); ^bsignificantly different from FITC/BSA‐P and RU486 (P < 0.01); ^csignificantly different from FITC/BSA‐P and RU486 (P < 0.05). Experiments were carried out four times using four hamsters.

Inline graphic — Effects of progesterone (P) on hamster sperm hyperactivation. (a) Significant increase with 20 ng/mL P. Values are means ± standard deviation. Modified Tyrode's albumin lactate pyruvate (mTALP) + 0.1% ethanol (EtOH) (vehicle, ), mTALP + 20 ng/mL P + 0.1% EtOH (P; ) and mTALP + 20 ng/mL P + 23.4 µmol/L RU486 + 0.1% EtOH (P and RU486, ). ^aSignificantly different from vehicle (P < 0.01); ^bsignificantly different from P and RU486 (P < 0.01). (b) Sperm hyperactivation significantly increased by P in a concentration‐dependent manner. Values are means ± standard deviation. Vehicle (), 10 ng/mL P (), 20 ng/mL P () and 40 ng/mL P (). ^aSignificantly different from vehicle (P < 0.01); ^bsignificantly different from 10 ng/mL P (P < 0.01); ^csignificantly different from 40 ng/mL P (P < 0.01); ^dsignificantly different from vehicle (P < 0.05). (c) Sperm hyperactivation increased by non‐genomic signals of P. Values are means ± standard deviation. Vehicle (), 20 ng/mL P (), mTALP + 7 nmol/L fluorescein isothiocyanate and bovine serum albumin conjugated progesterone + 0.1% EtOH (FITC/BSA‐P, ) and mTALP + 7 nmol/L FITC/BSA‐P + 23.4 µmol/L RU486 + 0.1% EtOH (FITC/BSA‐P and RU486, ). ^aSignificantly different from vehicle (P < 0.01); ^bsignificantly different from FITC/BSA‐P and RU486 (P < 0.01); ^csignificantly different from FITC/BSA‐P and RU486 (P < 0.05). Experiments were carried out four times using four hamsters.