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. 2008 Apr 17;7(2):63–74. doi: 10.1111/j.1447-0578.2008.00202.x

Figure 2.

Figure 2

Binding of progesterone (P) to sperm heads. (a,d) Hamster spermatozoa incubated in modified Tyrode's albumin lactate pyruvate (mTALP) with 3.5 nmol/L fluorescein isothiocyanate and bovine serum albumin conjugated progesterone (FITC/BSA‐P), which converted into approximately 10 ng/mL P, and 0.1% ethanol (EtOH). (b,e) Hamster spermatozoa incubated in mTALP with 7 nmol/L FITC/BSA‐P and 0.1% EtOH. (c,f) Hamster spermatozoa incubated in mTALP with 14 nmol/L FITC/BSA‐P, which converted into approximately 40 ng/mL P, and 0.1% EtOH. (g,i) Hamster spermatozoa incubated in mTALP with 7 nmol/L FITC/BSA‐P, 23.4 µmol/L RU486 and 0.1% EtOH. (h,j) Hamster spermatozoa incubated in vehicle. (a–c,g,h) Observed under a light field; (d–f,i,j) observed under a fluorescent field. Fluorescence of the mitochondria sheath in the flagellum was autofluorescence. Bar represents 100 µm.