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. 2008 Apr 17;7(2):63–74. doi: 10.1111/j.1447-0578.2008.00202.x

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© 2008 The Authors. Journal compilation © 2008 Japan Society for Reproductive Medicine

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Effects of phospholipase C (PLC) inhibitors on sperm hyperactivation. (a) U73122, which is an inhibitor of PLCδ, and U73343, which is a negative control of U73122, were added to the modified Tyrode's albumin lactate pyruvate (mTALP) medium. Values are means ± standard deviation. Vehicle (), 1 µmol/L U73122 (U73122, ), 1 µmol/L U73343 (U73343, ), 20 ng/mL P (P, ), 20 ng/mL P + 1 µmol/L U73122 (P and U73122, ) and 20 ng/mL P + 1 µmol/L U73343 (P and U73343, ). (b) D609, which is an inhibitor of phosphatidylcholine‐PLC (PC‐PLC), was added to the mTALP medium. Values are means ± standard deviation. Vehicle (), 10 µmol/L D609 (D609, ), 20 ng/mL P (P, ), 20 ng/mL P + 10 µmol/L D609 (P and D609, ). (c) ET‐18‐OCH3, which is an inhibitor of phosphatidylinositol‐PLC (PI‐PLC), was added to the mTALP medium. Values are means ± standard deviation. Vehicle (), 15 µmol/L ET‐18‐OCH3 (ET‐18‐OCH3, ), 20 ng/mL P (P, ) and 20 ng/mL P + 15 µmol/L ET‐18‐OCH3 (P and ET‐18‐OCH3, ). (d) Neomycine, which is a non‐specific inhibitor of PLC, was added to the mTALP medium. Values are means ± standard deviation. Vehicle (), 65 µmol/L neomycine (Neomycine, ), 20 ng/mL P (P, ) and 20 ng/mL P + 65 µmol/L neomycine (P and Neomycine, ). (e) Spermine, which is an inhibitor of PLCα and activator of PLCδ, was added to the mTALP medium. Values are means ± standard deviation. Vehicle (), 1 mmol/L spermine (Spermine, ), 20 ng/mL P (P, ) and 20 ng/mL P + 1 mmol/L spermine (P and Spermine, ). ^aSignificantly different from vehicle and inhibitor (P < 0.01); ^bsignificantly different from P and inhibitor (P < 0.01); ^csignificantly different from vehicle and inhibitor (P < 0.05); ^dsignificantly different from P and inhibitor (P < 0.05). Experiments were carried out four times using four hamsters.

Inline graphic — Effects of phospholipase C (PLC) inhibitors on sperm hyperactivation. (a) U73122, which is an inhibitor of PLCδ, and U73343, which is a negative control of U73122, were added to the modified Tyrode's albumin lactate pyruvate (mTALP) medium. Values are means ± standard deviation. Vehicle (), 1 µmol/L U73122 (U73122, ), 1 µmol/L U73343 (U73343, ), 20 ng/mL P (P, ), 20 ng/mL P + 1 µmol/L U73122 (P and U73122, ) and 20 ng/mL P + 1 µmol/L U73343 (P and U73343, ). (b) D609, which is an inhibitor of phosphatidylcholine‐PLC (PC‐PLC), was added to the mTALP medium. Values are means ± standard deviation. Vehicle (), 10 µmol/L D609 (D609, ), 20 ng/mL P (P, ), 20 ng/mL P + 10 µmol/L D609 (P and D609, ). (c) ET‐18‐OCH3, which is an inhibitor of phosphatidylinositol‐PLC (PI‐PLC), was added to the mTALP medium. Values are means ± standard deviation. Vehicle (), 15 µmol/L ET‐18‐OCH3 (ET‐18‐OCH3, ), 20 ng/mL P (P, ) and 20 ng/mL P + 15 µmol/L ET‐18‐OCH3 (P and ET‐18‐OCH3, ). (d) Neomycine, which is a non‐specific inhibitor of PLC, was added to the mTALP medium. Values are means ± standard deviation. Vehicle (), 65 µmol/L neomycine (Neomycine, ), 20 ng/mL P (P, ) and 20 ng/mL P + 65 µmol/L neomycine (P and Neomycine, ). (e) Spermine, which is an inhibitor of PLCα and activator of PLCδ, was added to the mTALP medium. Values are means ± standard deviation. Vehicle (), 1 mmol/L spermine (Spermine, ), 20 ng/mL P (P, ) and 20 ng/mL P + 1 mmol/L spermine (P and Spermine, ). ^aSignificantly different from vehicle and inhibitor (P < 0.01); ^bsignificantly different from P and inhibitor (P < 0.01); ^csignificantly different from vehicle and inhibitor (P < 0.05); ^dsignificantly different from P and inhibitor (P < 0.05). Experiments were carried out four times using four hamsters.