Figure 2.

Apoptotic processing of recombinant Xenopus caspases were inhibited by acidic solutions in Xenopus egg extracts. Interphase egg extracts in the absence or presence of 10 mM AA, 10 mM DHA, and 10 mM Ac were mixed with rabbit reticulocyte lysates containing ³⁵S‐radiolabeled recombinant Xenopus caspases and incubated at room temperature for 0 or 4 h. Samples were resolved by SDS‐PAGE and detected by image analyzer. For each caspase, full length form (FL) and processed fragments (Frg.) are indicated. In the case of xCasp‐3, a slow‐migrating form is indicated by an asterisk