Figure 1.

Levels of activated leucocyte cell adhesion molecule (ALCAM) were altered in ovalbumin (OVA)‐challenged wild‐type (WT) mice. (a) Experimental protocol for induction of food allergy in mice. Mice were sensitized with 50 µg of OVA and 10 µg of cholera toxin (CT) and challenged with 50 mg of OVA. Clinical scores (b), diarrhoea scores (c) and core temperature (d) were measured after the last challenge. (e) OVA‐specific immunoglobulin (Ig)E levels in serum of the mice. (f) Serum levels of ALCAM measured by enzyme‐linked immunosorbent assay (ELISA). (g) ALCAM mRNA was measured in the small intestine by real‐time polymerase chain reaction. (h) ALCAM protein levels were measured in serum and small intestine by Western blotting. Data are representative of three independent experiments (n = 3–4 for each group). *P < 0·05, **P < 0·01 versus phosphate‐buffered saline (PBS)‐challenged control mice. [Colour figure can be viewed at http://wileyonlinelibrary.com]