Figure 6.

Effects of protease inhibitors on the shedding of the tumour necrosis factor (TNF)‐induced membrane‐bound form of leucocyte‐associated immunoglobulin (Ig)‐like receptor‐1 (mLAIR‐1). MH7A cells pretreated with the lentiviral vector (LV)‐LAR‐1 lentiviral vector were stimulated with TNF‐α alone or in combination with various protease inhibitors for 24 h. The membrane‐bound form of LAIR‐1 was quantified by flow cytometry. Data are shown as the percentage of mLAIR‐1 expression compared to control cells (no inhibitor). Group 1: no effect; group 2: partly inhibited; group 3: totally inhibited; group 4: increased expression. Data are representative of three independent experiments. Statistical differences in mLAIR‐1 expression between groups treated with TNF‐α only and those treated in combination with the indicated inhibitors are indicated. *P < 0·05; **P < 0·01.