Figure 7.

Effects of lentiviral leucocyte‐associated immunoglobulin (Ig)‐like receptor‐1 (LAIR‐1) expression on the tumour necrosis factor (TNF)‐induced inflammatory factors and matrix metalloproteinases (MMPs) in fibroblast‐like synoviocytes (FLS). MH7A cells were subjected to quantitative polymerase chain reaction (qPCR) for determining mRNA levels of the inflammatory factors (a) and MMPs (b). Control cells were incubated with the media alone (untreated control and untreated LAIR‐1 groups). Lentiviral vector (LV)‐control or LV‐LAIR‐1 lentiviral vector‐infected MH7A cells were incubated with 10 ng/ml TNF‐α in 1% serum‐free media for 24 h (control TNF‐α and TNF‐α‐induced LAIR‐1 groups). Relative mRNA levels were normalized to glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) *P < 0·05 versus untreated control cells, #P < 0·05 versus control TNF‐α cells. Data were obtained from three independent experiments.