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. 2017 Dec 8;154(1):76–88. doi: 10.1111/imm.12860

Figure 4.

Figure 4

Dextran sodium sulphate (DSS) ‐induced colitis changed the splenic immune response in mice with alveolar echinococcosis (AE). Cells were firstly gated on lymphocytes by size and singularity followed by DAPI exclusion to identify live cells for further analysis. Live lymphocytes were gated on CD4 expression to first identify CD4+ T cells. Then phycoerythrin (PE) ‐labelled Foxp3, interleukin‐10 (IL‐10), IL‐4 and interferon‐γ (IFN‐γ) expression was identified as Foxp3+, IL‐10+, IL‐4+ and IFN‐γ + T cells within CD4+ T cells. (a) Frequency of Foxp3+ T cells within CD4+ T cells in spleen cells from each group. (b) Frequency of CD4+ IL‐10+ T cells in spleen cells from each group. (c) Frequency of IL‐4+ T cells within CD4+ T cells in spleen cells from each group. (d) Frequency of IFN‐γ + T cells within CD4+ T cells in spleen cells from each group. The corresponding isotype control antibodies were identically labelled as staining controls. Data represent mean ± SD of three independent experiments of a total of 15–18 mice in each group (five or six mice per group in each independent experiment). (e) Representative images of Foxp3+, IL‐10+, IL‐4+, IFN‐γ + T cells within CD4+ T cells in spleen cells from each group. Comparison between groups was performed using a one‐way analysis of variance with Bonferroni's multiple comparison post‐test for statistical analysis. *P < 0·006. NS, no significance.