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. 2017 Dec 8;154(1):76–88. doi: 10.1111/imm.12860

Figure 5.

Figure 5

Antigen‐presenting cell (APC) activation was attenuated in the spleen of mice with alveolar echinococcosis (AE) upon dextran sodium sulphate (DSS) ‐induced colitis. Cells were first gated on size and singularity followed by DAPI exclusion to identify live cells for further analysis. Live cells were gated on CD11b or CD11c expression to first identify CD11b+ and CD11c+ cells. Then phycoerythrin‐labelled CD80, CD86 expression was identified as CD80, CD86 frequency within CD11c+ or CD11c+ cells. (a) CD80 frequency within CD11c+ and CD11b+ cells, CD80 mean fluorescence intensity (MFI) within CD11b+ cells in spleen cells from each group. (b) Representative images of CD80+ within CD11b+ cells in spleen cells from each group. (c) CD86 frequency within CD11c+ and CD11b+ cells, CD86 MFI within CD11b+ cells in spleen cells from each group. (d) Representative images of CD86+ within CD11b+ cells in spleen cells from each group. Comparison between groups was performed using a one‐way analysis of variance with Bonferroni's multiple comparison post‐test for statistical analysis. *P < 0·004.