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. 2017 Dec 8;154(1):76–88. doi: 10.1111/imm.12860

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© 2017 The Authors. Immunology Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Co‐stimulation was attenuated in the spleen of mice with alveolar echinococcosis (AE) upon dextran sodium sulphate (DSS) ‐induced colitis. Cells were first gated on size and singularity followed by DAPI exclusion to identify live cells for further analysis. Live cells were gated on CD11b or CD11c expression to first identify CD11b⁺ and CD11c⁺ cells. Then phycoerythrin‐labelled CD40 expression was identified as CD40 frequency within CD11c⁺ or CD11c⁺ cells. (a) CD40 frequency within CD11c⁺ and CD11b⁺ cells, CD40 mean fluorescence intensity (MFI) in CD11b⁺ cells in spleen cells from each group. (b) Representative images of CD40⁺ within CD11b⁺ cells in spleen cells from each group. Comparison between groups was performed using a one‐way analysis of variance with Bonferroni's multiple comparison post‐test for statistical analysis. *P < 0·004.