Figure 1.

Activity of Escherichia coli produced recombinant glycodelin (rGd). (a) Jurkat cells were treated with the indicated concentration of rGd for 24 h. Cells were then stained with annexin V‐fluorescein isothiocyanate (FITC) and propidium iodide (PI) and the apoptotic population was measured by flow cytometry (Student's t‐test P < 0·0005). (b) The alloactivated PBMCs were pretreated with 0·67 μM and 1 μM rGd for 24 h, then added to (CFSE)‐labelled human hepatocellular carcinoma expansion media (HepG2e) cells at a ratio of 10 : 1 and incubated for 4 h. After incubation, cells were harvested, stained with PI and analysed by flow cytometry (Student's t‐test, P < 0·012). Data are representative of three independent experiments.