Figure 5.

Down‐regulation of genes associated with cytotoxic activity of alloactivated peripheral blood mononuclear cells (PBMCs) by recombinant glycodelin (rGd). RNA was isolated from the tumours after injection of AP or APG. Real‐time polymerase chain reaction (PCR) was performed to detect the mRNA levels of human (a) CD4, (b) CD8, (c) interleukin (IL)‐2, (d) granzyme‐B, (e) eomesodermin (EOMES), (f) tumour necrosis factor (TNF)‐α and (g) IL‐6. The inset table tabulates the fold change in mRNA levels of each protein in APG in comparison to AP on day 3. Data are represented as the fold expression relative to the day 3 AP for each protein (Student's t‐test; *P < 0·05; **P ≤ 0·01; ***P < 0·001). AP = alloactivated PBMCs; APG = alloactivated PBMCs treated with rGd.