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. 2018 Apr 11;6:39. doi: 10.3389/fcell.2018.00039

Figure 2.

Figure 2

Isolated oNCSCs form neurospheres and show stem cell characteristics in vitro. (A,B) Cells isolated from the ovine sub-palatal tissue form neurospheres in serum-free culture conditions. Scale bar: 100 μm. (C) Dissociated oNCSCs can be expanded in vitro as adherent monolayer cultures. Scale bar: 100 μm. (D) oNCSCs express mRNA for the typical neural-crest markers Nestin, Twist, and Slug. NTC: no template control. Actin: housekeeping gene. (E) oNCSCs isolated from different animals express similar levels of Nestin, (F) Twist and (G) Slug. Data is shown as mean ± SEM from three independent experiments, ANOVA with Bonferroni correction, *p < 0.5 was considered significant, CI 95%. (H) Immunocytochemistry revealed expression of Nestin (yellow) at protein level. A representative experiment is shown. Nuclear counterstaining was performed using DAPI (cyan). Scale bar: 50 μm.