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. 2018 Apr 11;6:39. doi: 10.3389/fcell.2018.00039

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Copyright © 2018 Zeuner, Didenko, Humphries, Stergiadis, Morash, Patel, Grimm and Widera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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oNCSCs are highly proliferative and migrate in vitro. (A) Total cell number was determined after 24, 48, 72, and 96 h in adherent cultures of oNCSCs isolated from three different animals revealing normal exponential growth. (B) No significant differences in the population doubling time was observed between different oNCSC isolations. (C) In vitro wound healing assay was performed to assess the migratory potential of oNCSCs. Representative images are shown. Scale bar: 500 μm. (D) Scratch coverage was assessed over time. Full coverage was achieved between 9 and 11 h. All data are presented as mean ± SEM from three independent experiments.