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. 2018 Apr 11;6:39. doi: 10.3389/fcell.2018.00039

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Copyright © 2018 Zeuner, Didenko, Humphries, Stergiadis, Morash, Patel, Grimm and Widera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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oNCSCs differentiate into cells of the ectodermal and mesodermal lineage. (A) oNCSCs were differentiated toward the ectodermal fate followed by immunocytochemical analysis. Cells subjected to ectodermal differentiation showed β-III-tubulin expression (middle panel). Nuclei were counterstained using DAPI. Scale bar: 50 μm. (B) Smooth muscle actin (αSMA) was expressed in oNCSCs after mesodermal differentiation. Nuclei were counterstained using DAPI. Scale bar: 50 μm.